Associations between threats and landscape features within each ecoregion and lake basin were based principally on the informed opinions of natural resource professionals because the scientific literature rarely presented information on regional susceptibility to threats by landscape features or wildlife species. Regional Technical Workshops were attended by natural resource professionals from State, Federal and local agencies, NGOs, and universities. Attendees were asked to identify significant threats to landscape features within their ecoregion or lake basin of expertise. A list of potential threats, based on identified threats to SGCN associated with the landscape feature, was used as a starting point for discussion.